Downloads
Resources available for download include 3-photon data sets and software tools.
​
Data set: near-simultaneous 2- and 3-photon excitation​​​
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
Downloads
Resources available for download include 3-photon data sets and software tools.
​
Data set: near-simultaneous 2- and 3-photon excitation​​​
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
Downloads
Resources available for download include 3-photon data sets and software tools.
​
Data set: near-simultaneous 2- and 3-photon excitation​​​
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
Downloads
Resources available for download include 3-photon data sets and software tools.
​
Data set: near-simultaneous 2- and 3-photon excitation​​​
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
Downloads
Resources available for download include 3-photon data sets and software tools.
​
Data set: near-simultaneous 2- and 3-photon excitation​​​
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
Downloads
Resources available for download include 3-photon data sets and software tools.
​
Data set: near-simultaneous 2- and 3-photon excitation​​​
Takasaki et al. (2020) generated near-simultaneous 2- and 3-photon
fluorescence movies to compare imaging modalities.
Movies are available for download from the CRCNS data sharing site.
GCaMP6s fluorescence.
910 and 1300 nm excitation.
400 x 200 µm field of view.
~3.6 Hz frame rate.
220-650 µm below the pial surface
of mouse primary visual cortex.
The resource for 3-photon excitation
Welcome to 3photon.org, an online repository of information about biological fluorescence microscopy with 3-photon excitation.
We aim to provide content of value to a wide range of scientists, from those looking to improve their understanding of the relatively new field of 3-photon microscopy, through experimentalists looking to convert a 2-photon microscope, to grizzled veterans looking for information on the latest developments.
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Please contact us with suggested improvements and subscribe to be notified when the site is updated.
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Information on 3photon.org is grouped into several categories, accessed via the menu bar.​
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Fundamentals: Key information about 3-photon excitation, including its performance and limits.
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​Hardware: Practical information on building, aligning and using a 3-photon microscope, including key hardware used in published studies.
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Resources: Online resources, including data sets and tools available for download, information on imaging conferences and workshops, and links to the many references cited throughout 3photon.org.
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Site: 3photon.org is under constant development. If there's information you'd like to see on our site - changes you'd like to suggest, perhaps, or new topics you'd like to read about - please contact us. We welcome feedback and suggestions.
